keywords: Alloxan, antidiabetic, chlopropamide Musa paradisiaca, hypoglycemic, wistar rats
The hypoglycemic potential and likely antidiabetic properties of the aqueous extract of unripe Musa paradisiacal (plantain) on blood glucose level of wistar rats was investigated and compared with known potent antidiabetic drug (chlorpropamide) in an attempt to encourage exploration of hidden food substances with medicinal properties. Thirty wistar rats were used and divided into six groups of five rats each. Group 1 served as the normal control (positive control) and Groups 2, 3, 4, 5 and 6 were administered with alloxan (100 mg/kg) intraperitoneally. Group 2 served as the diabetic control (negative control). Groups 3, 4 and 5 were orally administered with aqueous extract of Musa paradisiaca(140, 180 and 220 mg/kg) once daily for 14 days. Wistar rats in Group 6 were orally administered with chlorpropamide (84 mg/kg) once daily for 14 days. The serum concentration of glucose of all the rats in each group was determined 48 h after inducement of alloxan. This was counted as day one of the test and daily treatment was carried out according to the respective dosages of each group. The serum concentration of glucose of all the rats in each group was again determined after the 7th and 14th dose. There was significant (p<0.05) reduction of serum glucose in Groups 3, 4 and 5 that were administered with the aqueous extract of Musa paradisiaca after the 7th and 14th dose when compared to the negative control group. Group 6 that was treated with chlorpropamide (84 mg/kg) showed no significant (p>0.05) reduction of serum glucose compared to most effective dose of the aqueous extract (220 mg/kg) after the 7th and 14th dose. This result suggests that the aqueous extract of Musa paradisiacapossess some hypoglycemic potential and anti-diabetic effect on alloxan induced diabetic rats and thus could be recommended to diabetic patients.
Etuk EU 2010. Animals models for studying diabetes mellitus. Am. J. Clin. Nutri.,1(2): 130-134. Hoffbrand V & Moss P 2011. Essential Haematology. 6th Edn., John Wiley and Sons, p. 560. Jekayinfa SO, Ola FA, Afolayan SO & Ogunwale RO 2012. On-farm energy analysis of plantain production in Nigeria. Energy for Sustainable Devt., 16(3): 339–343. Jimmy EO & Okon MA 2012. Periodic Validation of High Antidiabetic Potentials of Unripe Plantain in Comparison with Glibenclamide and Fansidar. Am. J. Pharmac. &Toxico.,7(1): 15-18. Manders RJF, Wagenmakers AJM, Koopman R, &Zorenc AHG 2005. Co-ingestion of a protein hydrolysate and amino acid mixture with carbohydrate improves plasma glucose disposal in patients with type 2 diabetes. Am. J. Chin. Nutr., 82: 76-83. Niba LL2004.Beta-Glucan, Fructo-Oligosaccharide and Resistant Starch in Processed Plantain (Musa paradisiacaL.). J. Food Techn., 4: 216-220. Nwafor PA, Jack TW, Ekanem AU & Poh CF 2005. Antiulcerogenic and antidantidiarrhoeal potentials of methanolic extract of Pausinystalia Macroscera Stem-bark in rate. Nig. J. Nat. Prev. Med.,9: 63-67. Pi-Sunyer FX 2002. Glycemic Index and Disease.Am. J. Clin. Nutri.,76: 2905-2985. Srivastava UC & Kumar S 2011. Phytochemicals as cure of worm infections in traditional medicine systems. Emerging Trends in Zoology, Narendra Publishing House, p. 351–378. Sudagani J & Hitman GA 2013. Encyclopedia of Human Nutrition (3rd Edition), pp. 40-46. Szudelski TS 2001. The mechanism of alloxan and streptozotocin action in ß cells of the rat pancreas. Physiolo. Res.,50: 536-546. Takasu N, Komiyada I, Asawa T, Nagasawa Y & Yamada T 1991. Streptozotocin and alloxan induced H2O2 generation and DNA fragmentation in pancreatic islets. H2O2 as mediator for DNA fragmentation.Diabetes,40: 1141-114. Trease GE 1966. A Textbook of Pharmacognosy. 9th Edn.,Tindall and Cassell, London, p. 821. Williamson EM, Okpako DT & Evans FJ 1996. Pharmacological Methods in Phytotherapy Research. 1st Edn., John Wiley and Sons, Chicheser, p. 238. Lasselin J, Layé S, Dexpert S, Aubert A, Gonzalez C, Gin H & Capuron L 2012. Fatigue symptoms relate to systemic inflammation in patients with type 2 diabetes. Brain, Behavior & Immunity, 26(8): 1211–1219. Banerjee M & Vats P2014. Reactive metabolites and antioxidant gene polymorphisms in type 2 diabetes mellitus. Indian J. Human Genetics, 20: 10-19.