keywords: β-sitosterol, Cassytha filiformis, FT-IR, NMR, RumfarGada, stigmasterol
Cassytha filiformis, a leafless and perennial vine with small scales as a replacement of the leaves is currently being used in the treatment of various disease conditions such as jaundice, fever and malaise. Preliminary phytochemical screening of various extracts (Petroleum ether, Ethyl acetate and methanol) showed that the whole plant contains alkaloids, steroidal nucleus, phenolic nucleus, terpenoids, flavonoids and saponins. Isolated compound 5B from petroleum ether extracts was analyzed using various test ranging from physical (Melting point 144-146oC uncorrected and Solubility in ethyl acetate and chloroform), chemical (Positive reaction using Libermann-Buchard test) and spectroscopic techniques (NMR, FT-IR and MS) in order to determine the identity of the isolated compound. Based on the result obtained from these analyses and comparing the data with that reported in the literatures, 5B contained β-sitosterol and stigmasterol.
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