keywords: Diversity, DNA, genetic, heat, marker, QTL, wheat
Field trials were conducted at Lake Chad Research Institute Research Farm at Dadinkowa, Gombe State-Nigeria, during 2014/2015 and 2015/2016 dry seasons. The objectives of this study was undertaken to identify and evaluate heat tolerant lines and genetic diversity from bread wheat and to explore the potential of molecular markers in improving wheat heat tolerance. 24 wheat lines were used; heat stress was imposed through staggered sowing. Normal sowing (15th November) was non-stress and late sowing (6th January) resulted in terminal heat stress. The lines were laid out in Randomized Complete Block Design (RCBD) in triplicates during 2015/2016 dry season in plots measuring 3x2 m with 6 rows and 30 cm row spacing. The analysis of variance was computed using the General Linear Model (GLM) SAS version 9.2. Wizard Genomic DNA Purification Kit was used for DNA extraction. DNA was extracted by Cetyltrimethylammonium Bromide (CTAB) method. The result of means square indicated significant difference between genotypes. The results also indicated that the number of alleles range from 1- (Dreb-B1) to 9- (Xgwm577), genetic diversity index varied greatly among the loci from 0.0000 in case of Dreb-B1 to 0.8471 in case of Xgwm577. The Polymorphic Information Content (PIC) value were from 0.0000 (Dreb-B1) to 0.8296 (Xgwm577). The lowest and highest genetic distances level were 0.083 and 0.750, respectively. Cluster analysis had grouped the accessions into 5 groups at a genetic distance level of 0.15. In conclusion, genotypes 4404, 4408, 4410, 4411, 4413, 4414, and 4420 were identified as top yielder as such could be explored for resistance lines against heat stress.
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