(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170


Pages: 89-95

keywords: Bilirubin, carbon tetrachloride, hepatoprotective, Jaundice, Mucuna pruriens


This study investigated the bilirubin clearance and the hepatoprotective properties of the methanolic extract of Mucuna pruriens leaves in Carbon Tetrachloride (CCl4) induced albino rats. CCl4 was injected into the albino rats to induce jaundice; the rats were given oral dose (20, 40 and 60 mg/kg body weight) of methanolic extract of M. pruriens simultaneously. The effect of this treatment on liver enzymes, total bilirubin, total albumin, total protein, urea, creatinine and cholesterol were evaluated. The administration of CCl4 alone significantly (P less than 0.05) increased activities of serum, ALT, AST, ALP, GGT, total bilirubin, creatinine, cholesterol. The concentration of albumin and total protein were significantly reduced (P less than 0.05). Simultaneous treatment of CCl4 injection and oral administration of 20, 40 and 60 mg/kg body weight of the methanolic extract of M. pruriens significantly reversed (P less than 0.05) these changes in the serum. The above results suggest that the extract may probably possess component that has anti jaundice properties.


Ahmad I & Beg AZ 2001. Antimicrobial and Phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethnopharm., 74: 87-91. Ajit, Sarkar & Das AP 2010. Ethnobotanical formulations for the treatment of jaundice by the Mech tribe in Duars of West Bengal. Indian J. Trade Knowl., 9: 134 136. Akhtar MS, Qureshi AQ & Iqbal J 1990. Antidiabetic evaluation of Mucuna pruriens Linn seeds. J. Pak. Med. Assoc., 40(1): 147-150. Amin KMY, Khan, MN, Zillur-Rehman, S & Khan, NA 1996. Sexual function improving effect of Mucuna pruriens in sexually normal male rats. Fitoterapia Milano, 67(1): 53-56. Arias I, Wolkoff A, Boyer J, Shafritz D, Fausto N, Alter H & Cohen D eds. 2011. TheLiver Biology and Pathology. John Wiley and Sons, New Jersey, p. 1216. Baladrin MF, Clocke JA, Wurtele ES & Bolinge WH 1985. National Plant Chemicals: Source Indu. & Medicinal Materials Sci.,228: 1154-1160. Beatriz G, Talayero MD, Frank M & Sacks MD 2011. The Role of Triglycerides in Atherosclerosis. Curr. Cardiol. Rep., 13(6): 544–552. Bibitha B, Salitha VK, Mohan CV & Valsa AK 2002. Antibacterial activity of different plant extract. Short Communication. Indian J Microbio., 42: 361-363. Binu S 2009. Medicinal plants used for treating jaundice (Hepatitis) by the tribals in pathanamthitta district of kerala. J. Non-timber Forest Products, 16: 327-330. Boer HJ, Kool A, Broberg A, Mziary WR, Hedberg I & Levenfors JJ 2005. Antifungal & Antibacterial Activity of Some Herbal Remedies from Tanzania, 96: 461-469. Bopanna KN, Kannan J, Sushma G, Balaraman R & Rathod SP 1997. Antidiabetic and antihyperlipaemic effects of neem seed kernel powder on alloxandiabetic rabbits. IndianJ. Pharmaco., 29(3): 162-167. Bravo L, Siddhuraju P & Saura-Calixto F 1998. Effect of various processing methods on the in vitro-starch digestibility and resistant starch content of Indian pulses. J. Agric. & Food Chem., 46(1): 4667-4674. Carlson RV, Boyd KM & Webb DJ 2004. The revision of the Declaration of Helsinki: past, present and future. Br. J. Clin. Pharmacol., 57(6): 695-713. Dennery PA, Weng YH, Stevenson DK & Yang G. 2001. The biology of bilirubin production. J. Perinato., 21(1): 17-20. Gornall AG, Bardawill CJ & David MM 1949. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem., 177: 751-766. Goutam B, Dilip G & Rajiv 2013. Argemone Mexicana: Chemical and pharmacological aspects Rev. Bras. Farmacogn., 23: 3 Guerranti R, Aguiyi JC, Errico E, Pagani R, & Marinello E. 2001. Effects of Mucuna pruriens extract on activation of prothrombin by Echiscarinatus venom. J. Ethnopharmac.,, 75(1): 175-180. Guerranti R, Ogueli, IG, Bertocci E, Muzzi C, Aguiyi JC, Cianti R, Armini A, Bini L, Leoncini R, Marinello E & Pagani R 2008. Proteomic analysis of the pathophysiological process involved in the anti-snake venom effect of Mucuna pruriens extract. Proteomics, 8(1): 402-412. Gupta M, Mazumder UK, Chakraborti S, Bhattacharya S, Rath N & Bhawal SR 1997. Antiepileptic and anticancer activity of some indigenous plants. Indian J. Physio. & Allied Sci., 51(1): 53-56. Hamid O, Schmidt H, Nissan A, Ridolfi L, Aamdal S, Hansson J, Guida M, Hyams DM, Gómez H, Bastholt L, Chasalow SD & Berman D 2011. A prospective phase II trial exploring the association between tumor microenvironment biomarkers and clinical activity of ipilimumab in advanced melanoma. J. Translational Medicine, 28(9): 204. Hansen TWR 2000. Bilirubin oxidation in brain. Molecular Genetics & Metabolism, 71(1): 411-417. Kavitha C & Thangamani C 2014. Amazing bean Mucuna pruriens: A comprehensive review J. Medicinal Plants Res., 8(2): 138-143. Kinard G 2009. Grin taxonomy for plants. United States Department of Agriculture (USDA) and Agriculture Research Service (ARS).Available at: http;//www.ars-grin.gov/cgi-bin/npgs/html/taxgenform.pl. Accessed 1st May, 2015. KottaiMuthu A, Kumar DS, Smith AA & Manavalan R 2010. In vitro antioxidant activity of various extracts of whole plant of Mucuna pruriens (Linn). Int. J. PharmTech Res., 2(1): 2063-207. Lee HY, Bahn SC, Shin JS, Hwang I, Back K, Doelling JH & Ryu SB 2005. Multiple forms of secretory phospholipase A2 in plants. Progress in Lipid Res., 44(1): 52-67. Lorenzetti E, MacIsaac S, Arnason JT, Awang DVC & Buckles D 1998. The phytochemistry, toxicology and food potential of velvet bean (Mucuna aadans spp. Fabaceae). In: Buckles, D, Osiname, O, Galiba, M & Galiano, G. Eds. Cover crops of West Africa: Contributing To Sustainable Agriculture. IDRC, Ottawa and IITA, Ibadan, p. 318. Lusková V, Svoboda M & Kolá¤Ová J 2002. The Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.). Acta Vet. Brno, 71: 117-123 Malloy HT & Evelyn KA 1937. The determination of bilirubin with the photometric colorimeter. J. Biol. Chem., 119: 481-490