(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170


Pages: 814-817
M. M. Babatunde and Mariam Suleiman

keywords: Clariasgariepinus, degenerative inflammation, gastrointestinal tract, hemorrhage, necrosis, toxicant


The acute toxicity test of lead to Clariasgariepinus juveniles and the rate of bioaccumulation in the intestine of Clariasgariepinus juveniles was investigated by Standard Methods by APHA (2000). At the end of the 96 hours the gastrointestinal tract were rinsed in 10% formalin and preserved for further analysis. In this study, the LC50 values of Clariasgariepinus at 96 h period was determined to be 50.118 ppm or 50.12 mg/ L. Results obtained from this study showed that lead (Pb(NO3)2) has extensive histological changes in the gastrointestinal tract exposed to the various lead concentrations of 100 mgL-1, 75 mgL-1 and 50 mgL-1. The lead concentration disturbed the homeostasis and led to physiological disorders in their functions and subsequently to the death of the juveniles Clariasgariepinus. The effects of toxicant on the gastrointestinal tract of fish on this study ranged from slight changes in motility, secretion and absorptive functions to more severe effects associated with mucosal integrity, blood flow and impacted neuromuscular control.


Abdallah GM, El-Sayed SM &Abo-Salem OM 2010. Effect of lead toxicity on coenzyme Q levels in rat tissues. Food Chemical Toxicology, 48: 1753-1756. APHA 2000. Standard Methods for the Examination of Water and Wastewater. 21st edition Eaton, A.D.L.C. Clesceri and A.E. Greenberg (eds.). American Public Health Association, Washington DC. Babatunde MM 2008. Toxicity of galexoreochromisniloticus (Trawavas) proceedings of the 23rd Annual Conference of Fisheries Society of Nigeria (FISON), Kaduna State of Nigeria. Babatunde MM,BalogunJK, Oladimeji AA, Auta J &Balarabe ML2014. Variations of phytoplankton abundance and species composition in Kudiddifi-Kubani stream, Hanwa-Makera, Zaria, Nigeria. Implication for water quality. Int. J. Advance Sci. Technical Res. Bano Y &Hasan M 1990. Histopathological lesions in the body organs of catfish (Heteropneustesfossilis), following mercury intoxication.J.Envtal. Sci.&Health, 25(1): 67-85. Berntssen MHG, Hylland K, Wendelaarand BSE &Maage A 1999. Toxic levels of dietary copper in Atlantic salmon (Salmosalar L.).Aquatic Toxicology, 46: 87-99. Bhatnagar C, Bhatnagar M &Regar B 2007. Fluoride-induced histopathological changes in gill, kidney and intestine of freshwater teleost, Labeorohita. Resource Representation. Fluoride, 40: 55-6.CengizEI&Unlu E2006. Sub lethal effects of commercial deltamethrin on the structure of the gill, liver and gut tissues of mosquito fish, Gambusiaaffinis. A microscopic study.Envtal.Toxicol.Pharmaco., 21: 246-253. Chandravathy V &ReddySL 1996. Lead nitrate exposure changes in carbohydrate metabolism of freshwater fish. J. Envtal. Bio., 17(1): 75-79. DeFrancisco JD, Troya, R &AgüeraEI 2003. Review – Lead and lead toxicity in domestic and free living birds.AvianPathology, 32: 3-13. Delashoud M, Pousty I, Banan–Khojasteh SM2010. Histology of bighead carp (Hypophthalmichthysnobilis) intestine. Global Veterinary, 5:302-306. Diaz AO, Garcia AM, Figueroa DE&Goldemberg AL2008. The mucosa of the digestive tract in Micropogoniasfurmieri: A light and electron microscope approach. Anatomical Histology of Embryology, 37:251-256. Dorin D, Sire MF&Vernier J 1993. Endocytosis and intracellular degradation of heterologous protein by eosinophilic granulocytes isolated from rainbow trout (Oncorhynchusmykiss) posterior intestine. Cell Biology, 79:219-224. EIFAC (European Inland Fisheries Advisory Commission) 1983. Working party on toxic testing procedures revised report on fish toxicity testing procedures paper, EIFACTechnical Paper, 24:1- 37. EzeasorDN&StokoeWM 1981. The light and electron microscopic studies on the absorptive cells of the intestine, caeca and rectum of the adult rainbow trout, SalmogairdneriRich. J. Fisheries Bio., 18:527-544. FAO (Food and Agricultural Organization) 1981. Compilation of legal limits for hazardous substances in fish and fishery products. Fisheries circular No.764. FAO, Rome. Italy, 102p. farms on the water column nutrient concentrations and accumulation of heavy metals in the sediments in the eastern Aegean Sea (Turkey). Environmental Monitoring Assessment, 162: 439-451. Fish Toxicity Testing Frame Work. (2012). Environment, Health and Safety Publication Series on Testing and Assessments, p. 171. Haensly WE, Granger HJ, Morris AC &Cioffe C 1982. Promixal- tubule- like epithelium in Bowman’s capsule in spontaneously hypertensive rats: changes with age. Animal J.Pathology, 107: 92 – 97. Mahmoud SA &El-NaggarAM2007. Alterations in Clariasgariepinus caused by pollutants at El Rahawy area, Rosetta branch, River Nile, Egypt. J. Egyptian Acade.Envtal.Devt., 8(2): 61-70. Mirhashemi SM, MoshtaghieAA, AniM &AarabiMH 2010. Lead toxicity on kinetic behaviors of high and low molecular weight alkaline phosphatase isoenzymes of rat, in vivo and in vitro studies. J. Biol.Sci., 10: 341-347. Murty AS 1986. Toxicity of pesticide to fish. Volume II CRCPres Boca Raton, Florida. Nevin Rick 2000. How Lead Exposure Relates to Temporal Changes in IQ, Violent Crime, and Unwed Pregnancy. Environmental Research SectionA 83: 1-22. Academic Press. Niyogi S &WoodCM 2004. Biotic ligand model a flexible tool for developing site specific water quality guidelines for metals. Envtal. Sci. Techn., 38: 6177-6192. Ordija V 1993. Lessons from Lordship. Pages 73–79 in National shooting range Symposium Proceedings, 17–19 October. Salt Lake City, Utah. Otitoloju AA 2001. Joint Action Toxicity of Heavy Metals and Their Bioaccumulation by Benthic Animals of the Lagos Lagoon Ph.D Thesis University of Lagos. Otitoloju AA&Don Pedro KN 2002. Establishment of the Toxicity Ranking Order of Heavy Metals and Sensitivity Scale of Benthic Animals Inhabiting The Lagos Lagoon. West Afr. J. Appl. Ecol., 3, 31-41. Park JY& Kim IS 2001. Histology and mucinhistochemistry of the gastrointestinal tract of the mud loach in relation to respiration. Journal of Fish Bio., 58:861-872. Powell MD, Briand HA, Wright GM& Burka JF 1993. Rainbow trout (OncorhynchusmykissWalbaum) intestinal eosinophilic granule cell (EGC) response to Aeromonassalmonicida and Vibrio anguillarum extracellular products. Fish Shellfish Immunology, 3:279-289. Reglero MM, TaggartMA, Monsalve-Gonzalez &Mateo R 2009. Heavy metal exposure in large game from a lead mining area: effects on oxidative stress and fatty acid composition in liver. Environmental Pollution, 157: 1388-1395. Ribeiro L, Sarazquete C&Dinis MT 1999. Histological and histochemical development of the digestive system of Soleasenegalensis (Kaup, 1858) larve. Aquaculture, 171:293-308. Sanderson Glen C &Frank C Bellrose 1986. A Review of the Problem of Lead Poisoning in Waterfowl. Illinois Natural History Survey, Champaign, Illinois. Special Publication 4. 34pp. Jamestown ND: Northern Prairie Wildlife Research Center Online. Retrieved November 3, 2005 from http://www.npwrc.usgs.gov/resource/birds/pbpoison/pbpoison.htm (Version 17OCT97). Schlenk D &Benson WH 2005. Target organ toxicity in marine and freshwater teleosts, Volume 1: Organs, Taylor and Francis Group, Boca Raton, New York. Soufy H, Soliman MK, El-ManakhlyEM&Gaafar AY2007. Some biochemical and pathological investigations on monosex Tilapia following chronic exposure to carbofuran pesticides.Global Veterinary 1: 45-52. Tayel SI, IbrahimSA,AuthmanMN&El-KashefMA 2007. Assessment of sabal drainage canal water quality and its effect on blood and spleen histology of Oreochromisniloticus. Afr. J. Bio. Sci., 3(1):97-107. Tongo II 2010. Toxicological Studies on the Effects of Agricultural Pesticides (Endosulfan and Diazinon) on Adult Buforegularis (Reuss, 1833). Ph.D Thesis, University of Benin. 271pp.