(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170


Pages: 1020-1024
Michael Nosano Yakubu, Busayo Olalekan Olayinka and Gbonjubola Olusesan Adeshina

keywords: Antibiotics, biofilm, Klebsiella pneumoniae, resistance, susceptibility, uropathogens


Uropathogenic K. pneumoniae is one of the prominent uropathogens that plays a major rule in biofilm formation. Microbial biofilms pose a public health problem for the persons who require indwelling medical devices, as the microorganisms in the biofilms are difficult to treat with antimicrobial agents. This study determined the biofilm production and antibiotic susceptibility pattern of K. pneumoniae isolated from Ahmadu Bello University Teaching Hospital, Zaria. Isolates from urine samples submitted to the Medical Microbiology Laboratory of the hospital were collected and identified using standard microbiological techniques. The isolates were evaluated for their biofilm production potentials and their susceptibility to antibiotics was determined. From the 180 uropathogens isolated, 55 (37.93%) were K. pneumoniae. The result of biofilm production showed most of the K. pneumoniae to be biofilm producers (63.64%). The antibiotic susceptibility testing showed that, the isolates were generally resistant to amoxicillin (78.18%), chloramphenicol (69.09%), tetracycline (63.64), cotrimoxazole (60.00%), ceftriaxone (49.09), ceftazidime (47.30%), ciprofloxacin (47.27%) and gentamicin (40.00%) while being uniformly susceptible to meropenem (90.90%), amikacin (85.45%) and piperacillin-tazobactam (83.64). A total of 76% of the isolates had MAR indices ≥ 0.3 indicating the prior exposure of these uropathogens to these antibiotics and up to 74.55% of the K. pneumoniae were multidrug resistant. However, the difference between the multidrug resistance of the biofilm-positive and biofilm-negative K. pneumoniae isolates was not statistically significant (P< 0.05) in this study.


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