FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Combretum lamprocarpum, leaf, triterpenoid, lupeol, ethyl acetate, Combretaceae
The purpose of this work was to isolate and characterize the bioactive compound from the leaf of Combretum lamprocarpum Diels (Combretaceae). The powdered plant material was extracted using Microwave-Assisted Extraction technique and n-hexane, chloroform, ethyl acetate, and methanol as solvents. The ethyl acetate extract was loaded on a silica gel column using n-hexane and ethyl acetate as eluents. A pure compound was isolated and its Infra-Red, proton and carbon 13 Nuclear Magnetic Resonance spectra were measured. A pair of broad singlet protons appeared in the proton Nuclear Magnetic Resonance spectra at δ 4.54 and 4.67 (1H each). This was indicative characterization of the exo-methylene group present in the Lupane type of triterpenoids. The compound was characterized as Lupeol by comparing its carbon 13 Nuclear Magnetic Resonance spectroscopic data to the literature data. A compound, Lupeol (Lup-20(29)-en-3β-ol) was isolated from the ethyl acetate extract of the leaf of Combretum lamprocarpum. Thorough literature search shows that this compound was isolated from this plant for the first time.
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