keywords: Antimalarials, Boswellia dalzielli, SYBRGreen 1 assay, Plasmodium falciparum 3D7 strain
The antiplasmodial activity of Boswellia dalzielli (Burseraceae) Hutch leaves 70% aqueous methanol crude extract and its C-18 resin flash chromatography fractions were evaluated. The study was carried out using the SYBRGreen 1dye assay in an in vitro culture of parasitized red blood cells. Microscopy was implored to serve for validation of the results obtained by the SYBRGreen dye 1 assay. The leaf crude extract showed a poor bioactivity with an IC50 of 47 µg/ml against the 3D7 strain of Plasmodium falciparum. However, upon fractionation, one of the fractions gave an IC50 of 17.8 µg/ml activity but with a correspondingly high level of haemolytic effect. The leaves of Boswellia dalzielli showed no cytotoxicity against the liver carcinoma cell line (HUH-7) but it was highly lytic against the Red blood Cells. This attributes shows it’s none selectivity in its bioactivity against the Plasmodium parasites. Thus, having a very low therapeutic index and as such not a promising drug candidate against malaria, except if used in synergy with other compounds that could buttress its antiplasmodial activity and attenuate its haemolytic effect. Its GC-MS fingerprint displays an array of rich phytocompounds that could be useful to man and other organisms for the improvement of life conditions.
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