keywords: Antimicrobial activity, Commiphora africana root, crude extract, fractionation, phytochemicals
Commiphora africana is used in traditional medicine for the treatment of various ailments by the natives of northern Nigeria. The air-dried roots were extracted with 95% ethanol using a Soxhlet apparatus to obtain a crude extract. The crude extract obtained was successively partitioned into alkaloid, acid, polar and non-polar fractions. The crude extract and the different fractions were evaluated for phytochemicals and antimicrobial activity. The result of the preliminary phytochemical screening of the crude extract showed that saponins, tannins, reducing sugars, flavonoids, cardiac glycosides, triterpenoids, alkaloids and carbohydrates were detected in the root. The antimicrobial activity of the crude and different fractions was determined against some commercially important microorganisms. The crude extract and fractions exhibited characteristic strong concentration-dependent activity (CDA) against the test organisms, with zones of inhibition of 11-33 mm depending on the concentrations. The result of this study confirmed the documented records of traditional medicinal uses of Commiphora africana root.
Akiyama H, Kazuyasu F, Yamasaki O, Oono T & Iwatsuki K 2001. Antibacterial action of several tannins against Staphylococcus aureus. Journal of Antimicrobial Chemother, 48: 487‐491. Balandrin MF 1996. Commercial Utilization of Plant-derived Saponins: An Overview ofmedicinal, Pharmaceutical and Industrial Applications, In: Waller GR & Yamasaki K (eds) Saponins Used in Food and Agriculture: Plenum Press, pp. 1 – 14. Calixto JB 2000. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (Phytotherapeutic agents). Braz. J. Med. Biol. Res., 33: 179-189. Clarkson JJ, Chase MW & Harley MM 2002. Phylogenetic relationships in Burseraceae based on plastid rps 16 intron sequences. Kew Bulletin, 57(1): 183-193. Ezekiel I, Mabrouk MA & Ayo JO 2010. Study of the effect of hydro-ethanolic extract of Commiphora africana (Stem-bark) on sleeping time and convulsion in mice. Asian J. Medical Sci., 2(3): 81-84. Farnsworth NR, Blowster RN, Darmratoski D, Meer WA & Cammarato LV 1967. Studies on Catharanthus alkaloids IV Evaluation by means of TLC and ceric ammonium sulphate spray reagent. Lloydia, 27: 302-314. Hadissa T & Jean-Pierre D 2005. Use of Medicinal plants for the treatment of oral diseases in Burkina Faso. Journal of Ethnopharmacology, 104: 68-78. Harbone JB 1984. Phytochemical Methods, 2nd edition, Chapman and Hall Publications, London, New York, p. 288. Heatley NG 1944. A method for the assay of penicillin. Biochem. J., 38: 61-65. Hutchings A, Scott AH, Lewis G & Cunningham A 1996. Zulu medicinal plants aninventory. University of Natal Press, Pietermaritzeburg, South Africa. In the Leaf Alkaloid Content of Androgenic Diploid Plants of Datura innoxia. Planta Med., 54: 14-17. Isyaka M & Okwute S K 2013. Phytochemical, IR spectral and biological studies on the leaf extracts of Commiphora africana (Burseraceae). ChemSearch Journal, 4(1): 14-17. Jorgensen JH & Ferraro MJ 2009. Antimicrobial susceptibility testing: A review of general principles and contemporary practices. Clin. Infect. Dis., 49: 1749-1755.