keywords: Miscarriage, Escherichia coli, endocervical smear, urine, upland
Escherichia coli infection is a serious health threat to the ordinary people, especially the intending mothers all over the world, but more so in the developing countries. In this cohort study, 300 Endocervical swabs (ECS) and mid-stream urine (MSU) samples were collected within 8 hours to 24 h of miscarriages to determine the prevalence and role of Escherichia coli in pregnancy related miscarriages in the Upland Area of Rivers state, in this era of frequent miscarriages amongst Women. The ECS were cultured on chocolate, blood and MacConkey agar while MSU samples were inoculated on MacConkey and CLED media, respectively. The growths were isolated and characterized using biochemical activities including fermentation, Gram reaction, motility, colonial appearance, media selection. ECS samples were also screened for Chlamydia trachomatis using Chlamydia Rapid Test Device kit. Of the 130 isolates 54(41.5%) of them were Escherichia coli while the proportions of the infected women were highest within the 18-20 years and 21 – 25 years age cohorts (P<0.05). The prevalence of Escherichia coli showed significant relationship (p<0.05) with the illiteracy level of the women, more so, the level of this result statistically (p<0.05) associates Escherichia coli with miscarriages in the Upland area of Rivers state of Nigeria, and this calls for mass literacy and awareness among the intending mothers.
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