keywords: Biological, anti-oxidant, Cochlospermum planchonii, Cochlospermum, IC50, scavenging
This work was aimed at evaluating the biological and anti-oxidant potential of the ethyl acetate and methanol fractions of the root of Cochlospermum planchonii and also to carryout biological activities on petroleum ether, Chloroform ethyl acetate and methanol. Chloroform gave the highest extractive value followed by methanol, petroleum ether and ethyl acetate. The antimicrobial screening of the root extracts of the plants were carried out on the following Microorganisms; Staphylococcus aureus, Escherichia Coli, Salmonella Typhi, Shigellia Dysenteric, Microsperum Canis, and Candida albicans. Klebselia pneumonia, Vibrio Cholerae and Trichophyton sp to determine their activities. The root extract showed that seven (7) of the microorganisms were sensitive to all the four extracts and two (2) were resistant to the extracts and the zone of inhibition observed for the microbes. The ethyl acetate extract show the highest zone of inhibition ranging from 24 to 28 mm and petroleum ether extract show the lowest values ranging from 16 to 18 mm. The antioxidant activities of the extracts have a high concentration which gives high percentage of DPPH inhibition with low inhibitory concentration (IC50) value, of 1.06 µg/ml and 2.61 µg/ml for Methanol and ethyl acetate extracts respectively while the standard ascorbic acid have IC50 of 107.08 µg/ml as calculated from regression equation from the graph which means that the extracts were more active than the standard ascorbic acid in antioxidant properties as compared with standard ascorbic acid.
Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM & Farombi EO 2007. Phytochemical constituents and antioxidant activity of extract from the leaves of the Ocimum graticcimum. Sci. Res. Essay, 2: 163 - 166. Barry AL & Thornsberry C 1985. Susceptibility tests, diffusion test procedure. J. Chem. Pathol., 19: 492-500. Bauer AW, Kirby WMM, Sherris JC & Truck M 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45(4): 493-496. Burton GW & Ingold KU 1989. Mechanisms of antioxidant action: preventive and chain-breaking antioxidants, In J. Miquel, A.T. Quintanilla, & H. Weber (Eds.). Handbook of free radicals and antioxidants in biomedicine (Vol. 2) Boca-Raton, FL: CRC Press, pp. 29. Carini R, Poli G, Diazini MU, Maddix SP, Slater TF & Chessman KH 1990. Biochemical Pharmacology, 39: 1597-1601. Ghani A 1990. In Traditional Medicine. Jahangirnagar University, Savar, Dhaka: p. 15 – 40. Isah Y, Ndukwe IG & Ayo RG 2013. Phytochemical and antimicrobial analyses of stem-leaf of Cochlospermum planchonii. Journal of medicinal and herbal Therapy Res., 1: 13-17. www.bluepenjournals.org/jmphtr. Sadhu SK, Okuyama E, Fujimoto H & Ishibashi M 2003. Seperation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and anti-oxidant activities. Chem Pharm Bull., 51: 595-598. Saha K, Lajis NH, Israf DA, Hamzah AS, Khorizah, S, Khamis S & Syahida A 2004. Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J. Ethnopharmacol., 92: 263 – 267.