keywords: Flavonol, quercetin, quercetin-3-O-β-D-glucopyranoside, Trichilia emetica, column chromatography
The powdered stem bark of Trichilia emetica (Family: Meliaceae) was extracted with methanol. The resulting methanol extract was dissolved in water and successively partitioned against equal volume of chloroform and ethyl acetate. The ethyl acetate fraction was subjected to silica gel column chromatography and eluted with chloroform and chloroform: methanol in various ratios, with further re-chromatography of some eluates and final purification with flash chromatographic system with linear gradient of methanol/water (containing 0.1% formic acid) at a flow rate of 18 mL/min. Two pure compounds were isolated, and was structurally identified on the basis of 1D- and 2D- NMR experiments, and MS data analyses. Also, the data obtained were directly compared to those reported in the literature. It was concluded that the compounds isolated were quercetin and quercetin-3-O-β-D-glucopyranoside.