FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Antitrypanosomal, Combretum molle, aqueous and methanolic extracts, nagana
In this study, aqueous and methanolic fractions of Combretum molle were investigated in vitro for antitrypanosomal potentials against Trypanosomes. The test involved incubating the parasites, Trypanosoma brucei brucei, with (10 mg, 5 mg/ml) of the fractions in 96-well microtitre plate. Cessation or drop in the parasite motility, determined microscopically, was taken as a measure of efficacy of the extract against control wells of Diminazene aceturate at (3.5 mg/kg). The test organisms were immobilized within 40-50 minutes of incubation. The in vitro activity of the extract at (10 mg/kg and 5 mg/kg) for both methanolic and aqueous fractions containing saponins and flavonoids, respectively were also determined. The phytochemical constituents of the crude extracts revealed the presence of tanins, saponins, flavonoids, glycosides, cardiac glycosides, anthraquinones and carbohydrates, while alkaloids were not detected hence a need for continued and sustained research in ethnomedicinal plants.
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