(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170


THE AMINO ACIDS COMPOSITION OF SMOOTH LOOFAH (Luffa cylindrical L.), ROSELLE (Hibiscus sabdariffa) AND SESAME (Sesamum indicum) SEEDS
Pages: 85-88
H. O. Adubiaro, E. M. Ogunbusola and A. A. Olaleye

keywords: Amino acids, analysis, composition, roselle, sesame, smooth loofah


The amino acid composition of smooth loofah, roselle and sesame seeds were determined using standard analytical techniques. The amino acid analysis revealed that all the samples contained nutritional useful quantities of most of the essential amino acids (EAA). The total amino acid values were high at 97.2 g/100g (smooth loofah); 72.8 g/100g (roselle); 97.5 g/100g (sesame). The total essential amino acid (TEAA) ranged from 34.7 g/100g in roselle to 44.6 g/100g in sesame and 32.1 g/100g in smooth loofah and 42.0 g/100g in sesame with and without histidine, respectively. The most concentrated amino acid for the three seeds was glutamic acid wiyh values range from 15.7 g/100g protein in roselle to 31.4g/100g cp in smooth loofah. The predicted protein efficiency ratio (P-PER) range was 1.6 – 2.1. The isoelectric point (pI) ranged from 4.2 in roselle to 5.8 in sesame, showing the samples protein to be in acidic medium of the pH range. In the amino acid scores, limiting amino acids were serine (0.165) in smooth loofah, and lysine (0.435 and 0.468) in roselle and sesame, respectively in whole hen’s egg; Lys in all (0.25-0.53) in provisional EAA scoring pattern; only roselle and sesame would supply virtually the required EAAs for the pre-school child as most determinations were above 100% requirement. Isoleucine had the highest score in smooth loofah 1.75 g/100g and roselle (1.15 g/100g) while Met+Cys had the highest score in sesame seed (1.34 g/100g).


Adeyeye EI 2004. The chemical composition of liquid and solid endosperm of ripe coconut. Oriental J. Chem., 20 (3): 471 – 476. Adeyeye EI 2005. Amino acid composition of variegated grasshopper, zonocerus variegation. Trop, Sci., 45(4): 141-143. Adeyeye EI & Adamu AS 2005. Chemical composition and food properties of Gymnarchusniloticus (Trunk Fish). Biosci. Biotechnol. Rese. Asia, 3(2): 265 – 272. Adeyeye EI 2006. Amino acids composition of fermented African locust bean (Parkia biglobosa) seeds. J. Appl. & Envtal. Services, 2: 154 – 158. Adeyeye EI 2009. Amino acid composition of three species of Nigerian fish: Claria anguillaris, Oreochromis niloticus and Cynoglossus senegalensis. Food Chem., 113: 43-46. Alsmeyer RH, Cunnngham AE & Happich ML 1974. Equations to predict PER from amino acid analysis. Food Technol., 28: 34-38. Barampama Z & Zimard RE 1993. Nutrient composition, protein quality and antinutritional factors of some varieties of dry beans (Phaseolus vulgaris) grow in Burundi. Food Chem., 47: 159-167. Belavady B & Gopalan C 1969. The role of lysine in the pathogenesis of canine black tongue and pellagra. Lancet, 2: 956 – 957. Bingham S 1977. Dictionary of Nutrition. Barrie and Jenkins, London. Chavan UD, Mckenzie DB & Shahidi F 2001. Functional properties of protein isolates from beach pea (Lathyrus maritmus L.). Food Chem., 74: 177-187. FAO 1995. Sorghum and Millets in Human Nutrition FAO Food Nutrition series No 27, pp 76-84, Food and Agriculture Organization of the United Nations, Rome. FAO/WHO 1990. Protein Quality Evaluation. Report of Joint FAO/WHO consultation held in Bethesda, USA, 4-8 December, 1989, p. 51; FAO, Rome. FAO/WHO 1991. Protein Quality Evaluation, Report of Joint FAO/WHO expert consultation. FAO Food Nutrition Paper 51, pp 4-66; FAO/WHO, Rome. FAO/WHO/UNU 1973. Energy and Protein Requirements. WHO Technical Report Series No. 52, pp. 1-118; WHO, Geneva. FAO/WHO/UNU 1985. Energy and Protein Requirements. WHO Technical Report Series No. 724, pp. 13-205, WHO, Geneva. Ghafoorunissa MA& NarasingRao BS 1973. Effect of luecine on enzyme of the tryptophan –niacin metabolic Pathway in rat liver and kidney. Biochem. J., 134: 425-430. Mendoza C 2002. Effect of genetically modified low phytic and plants on mineral absorption. Int. J. Food Sci. Technol., 37: 759-767. Olaofe O & Akintayo ET 2000. Prediction of isoelectric point at legume and oil seed protein from their amino acid composition. J. Technosci., 4: 49-53. Oshodi AA, Olaofe O & Hall GM 1993. Amino acid, fatty acid and mineral composition of pigeon pea (Cajanus cajan). Int. J. Frod Sci. Nutr., 43: 187-191. Overzon O 2005. Roselle: The dawn of a sunrise industry. Online: http//www.gvezon.org/roselle. Oyarekua MA & Eleyinmi AF 2004. Comparative evaluation of the nutritional quality of corn, sorghum and millet ogi, prepared by modified traditional technique. Food Agric Environ., 2(2): 94-99. Paul AD, Southgate AT & Russel J 1976. First supplement to McCance and Widdowson’s the composition of foods. HMSO, London. Rost TT & Weier TE 1979. Botany. A brief introduction to plant biology. New York, p. 319. Salleh AL & Jassir M 1992. Chemical composition and microflora of black cumin (Nigella satira L.) seeds growing in Saudi Arabia. Food Chemistry,45: 139-242. Salunkhe, D.K. and Kadam, S.S. (1989). Handbook of World Food Legumes, Nutritional Chemistry, Processing Technology and Utilization. Boca Raton, CRC Press, Florida. Sandstrom B, Almggren A, Kivisto B & Cederblad A 1989. Effect of protein and protein source on zinc absorption in humans. J. Nutr., 119: 48-53. Tsai PJ, Me/nxosh J, Pearce P, Camden B & Jurdan BR 2002. Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L.). Extract Food Res. Int., 35: 351-356. White A, Hander P & Smith EI 1973. Principles of Biochemistry, 5th ed. McGraw Hill Kogakusha Ltd., Tokyo.