keywords: Drug loading, doxorubicin, methotrexate, composite, hydroxyapatite, sodium alginate
Drug delivery systems alter the pharmacodynamics and pharmacokinetics of the drug by modifying the rate at which the drug is being released into the system. Its success depends on the drug loading method employed. Four different methods (methods 1, 2, 3 and 4) of loading doxorubicin (DOX) and methotrexate (MTX) on hydroxyapatite-sodium alginate composite were investigated in this study. Doxorubicin was loaded well (above 80%) by all the four loading methods studied, while for methotrexate method 2 and 4 were better (39.98% and 37.10%, respectively) than method 1 and 3 (10.39% and 15.21%, respectively). Release study for doxorubicin, indicated that adsorption method (method 1) had faster release rate than other methods, and followed first order release rate with Fickian diffusion as the predominant release mechanism; while methods 2, 3 and 4 also followed first order release rate, but with a mixture of diffusion and degradation as the release mechanism. For methotrexate, only method 4 sustained the release of the drug for about 9 h, while other methods had high burst release effects, which provides insufficient release data for further kinetic study. These observations show that the success of a delivery system depends on the loading method.
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