FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Carbohydrate, co-culture, fungi, monoculture, rice husk, sugar, treatment.
Fungi isolates were obtained from decomposing rice husk using potato dextrose agar (PDA). These isolateswere Aspergillus fumigatus (AF), Aspergillus niger(AN), Aspergillus oryzae (AO), Trichophyton mentagrophyte (TM), Trichophyton rubrum (TR) and Trichophyton soudanense (TS). Pure strain of each fungal culture was obtained and identified. Measured quantities of freshly processed rice husk in Mandle’s medium were heat pre-treated in an autoclave at 121oC for 20 minutes. The isolated fungi as monoculture and co-culture combinations were inoculated into each of the pre-treated rice husks with the exception of two controls (heated and non fungal treated rice husk -C1 and non-heated and non-fungal treated rice husk -C2). A total of 23 rice husk treated groups were hydrolyzed for seven days and total carbohydrate, reducing sugar and non-reducing sugar contents were estimated. Data analysis was by analysis of variance (ANOVA). The rice husks treated with Trichophyton soudanense and Trichophyton rubrum (TS + TR) co-culture; Aspergillus fumigatus (AF) monoculture; Aspergillus oryzae and Aspergillus niger (AO + AN) co-culture yielded the highest carbohydrate contents of 20.53 ± 2.73 %, 19.52 ± 10.05 % and 18.80 ± 3.59%, respectively. The highest soluble reducing sugar contents of 2.66 ± 0.14 % and 2.61 ± 0.30 % were obtained from the rice husks treated with monocultures of Trichophyton mentagrophyte (TM) and Aspergillus fumigatus (AF), respectively. The highest soluble non reducing sugar contents of 18.08 ± 2.61 % and 16.92±9.75 % were obtained from the combination of Trichophyton soudanese and Trichophyton rubrum (TS+TR) and Aspergillus fumigatus (AF) treated rice husks, respectively.
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