keywords: Genetic variance, heritability, mutagen, sesame
An evaluation of the mutagenic efficiency and mutagenic effectiveness of fast neutron and sodium azide to induce genetic variability and beneficial mutation on Sesamumindicumwas carried out.The seeds of sesame were subjected to four treatments (0.5mM, 1.0mM, 1.5mM and 2.0mM) of sodium azide. The treatments were laid out in a completely randomized block design. Harvested seeds from M1generation were sown to raise the M2. The mutagenic effectiveness and efficiency of sodium azide in sesame was 2.27% and 0.72%, respectively. Mutagenic effectiveness was dose dependent. A nonlinear decline in mutagenic efficiency was observed with increasing concentration and dose of sodium azide. Upon comparism with the control, sodium azide induced beneficial variabilities on the agronomic traits evaluated at M2generation. Best growth recorded in most of the morphological and yield traits evaluated was at 0.5mM concentration of sodium azide concentration. Broad sense heritability estimates for the agronomic traits evaluated ranged from 1.11% to 97.82%. High heritabilities obtained in days to flowering (97.82%) and thousand seed weight (87.50%) indicates that variation for these characters are due to high additive gene effects and consequently the scope for improving yield through selection for these traits in sesame is high.
Adamu AK, Chung SS &Abubakar S 2004. The effect of ionizing radiation (Gamma rays) on tomato (LycopersiconesculentumL.). Nig. J. Experim. Bio., 5(2): 185-193. Ashri A 2007. Sesame (Sesamumindicum L.). In: R.J. Singh, Ed., Genetic Resources, Chromosome Engineering, and Crop Improvement, Vol. 4, Oilseed Crop, pp. 231-289, CRC Press, Boca Raton, FL,USA. Attere AF 1999. Preface. In: ChweyaJ.A. and EyzaguirreP.B. (Eds.). The Biodiversity of TraditionalLeafy Vegetables: IPGRI Rome, pp. 6-7. Bedigian D &Adetula OA 2004. CeratothecasesamoidesEndl. In: GrubbenGJH&Denton OA (Editors). PROTA 2: Vegetables/Légumes. PROTA, Wageningen, Netherlands. Bind, D., Dwivedi, V. K. and Singh, S. K. (2016). Induction of Chlorophyll Mutations through Physical and Chemical Mutagenesis in Cowpea (Vignaunguiculata(L.) Walp.). Int. J. Advanced Res., 4(2): 49-53. Chweya JA &EyzaguirrePB 1999. The Biodiversity of Traditional Leafy Vegetables. IPGRI, Rome, Italy, pp. 51-83. Falusi OA, SalakoEA&Funmi FM 2012. Inheritance of hairiness of stem and petiole in a selection from loca (Nigeria) germoplasm of sesame. Tropicultura, 20: 156-158. GiriSP&Apparao BJ 2011. Studies on effectiveness and efficiency of ethyl methannesulphate in pigeon pea. Bioscience Discovery, 2(1): 55-98.Godfray H, Charles John R. 2010. Food Security: The challenge of feeding 9 billion people. Science, 327: 812-818. Grace K, Daniel O, Anne WTM, Martha M, Mariuki J, MowoGJ&Jammdars R 2014. Genetic Variability and divergence of seed traits and seed germination of five provenances of Fadherbiaalbida. Afr. J. Plant Sci., 8(11): 482-491. Konzak CF, Nilan RA, Wagner J & Faster RJ 1965. Efficient chemical mutagenesis. In: Use of induced mutations in plant breeding. Radiation Botany, 5: 49 – 70. Kulthe M, Umesh P, Mogle NG &Kashid 2013. Study of mutagenic efficiency of NMU in winged bean (Psophocarpustetragonolobus(L.) DC.) Bioscience Discourse., 4(1): 121-123. Lee SY, Cheong JI& Kim TS 2003. Production of double haploids through anether culture of m1 rice plants derived from muatgenesis of fertile egg cells. Plant Cell Reports, 22: 218-223. Mahmoud MA, Khidir MO, Khalifa MA, El AhmadiAMB, MusnadHAR& Mohammed El TI 1995. Sudan: Country report to the FAO international technical conference on plant genetic resources (Leipzig, 1996), Khartoum, Sudan, pp. 1-6. Maundu PM, NjiroEI, Chweya JA, ImungiJK&Seme EN 1999. Kenya. In: Chweya JA &EyzaguirrePB (eds.): The Biodiversity of Traditional Leafy Vegetables. International Plant Genetic Resources Institute, Rome, Italy. MensahJK& Tope 2007. Performance of Mungbean 9 Vignamungo L. ( HEPPER) grownin mid-west Nigeria. America-Eurasian journal of Agricultural &Envtal. Sci., 2(6): 696-701. Moazzami AA, Andersson RE & Kamal-Eldin A 2006. Characterization and analysis of sesamolinoldiglucoside in sesame seeds. Biosci., Biotech. &Biochem.,70: 1478-1481. Mohammad P, kagar, H., Ahmadi, M, and Kesmaty M. (2009). Intraperitoneal injection of sesame oil inmice on Fear conditioning. J. Animal Bio. Res., 4: 39 – 33. Moll RH, Robinson FH&Cockerham CC 1960. Genetic variability in advanced generation of across of two open-pollinated varieties of Corn. Agronomy Journal 52: 171-173. Monica, S. and Seetharaman, N. (2016). Effect of Gamma irradiation and ethyl methane sulphonate (EMS) mutagenesis in early generation of Garden bean (Lablab purpureus(L.) Sweet var. typicus). Int. J. Advanced Scientific & Technical Res., 6(3): 2249-9954. Parry MAJ, Flexas J & Medrano H 2005. Prospects for crop production under drought: research priorities and future directions. Annual Applied Biology, 147: 211-26. Pearce RB, Mock JJ& Bailey TB 1979. Rapid method of estimating leaf area per plant in maize. Crop Science, 15: 691-694. Pham DT, Nguyen TTD, Carlsson AS & Bui MT 2010. Morphological evaluation of sesame (Sesamumindicum L.) varieties from different origins. Australian J. Crop Sci., 4(7): 498- 504. Prasad R 2002. Text Book of Field Crops Production, Indian Council of Agricultural Research, New Delhi, p. 821. Reynolds M, FoulkesMJ, Slafer GA, Berry P, Parry MAJ, SnapeJW& Angus WJ 2009. Raising yield potential in wheat. J. Experim. Botany, 60: 1899–18. Richards RA 2000. Selectable traits to increase crop photosynthesis and yield of grain crops. J. Experim. Botany, 51: 447–58. Samiullah K, WaniMR&Parveen K 2004. Induced genetic variability for quantitative traits in Vigna radiate (L) wilczek. Pak. J. Botany,36: 845-850. Singh RK&Chaudhary BD 1985. Biometrical methods in Quantitative Genetic analysis. Kalyani Publishers, New Delhi India, pp. 253-260. Statistical Analysis Software (SAS) 2004. SAS/STAT 9.1 User’s guide. SAS Institute Inc., Cary, NC. Uzun B &Cagirgan MI 2009. Identification of molecular markers linked to determinate growth habit in sesame. Euphytica, 166(3): 379-384. Wani AA 2009. Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate and their combination treatments inchickpea (Cicerarietinum L.). Asian J. Plant Sci., 8(4): 318 - 32. Weinberger K &Msuya J 2004. Indigenous vegetables in Tanzania: Significance and Prospects. Technical Bulletin No. 31. Shanhua: AVRDC. Weiss EA 1983. Oil Seed Crops. Longman, London, New York, 660pp. Zaka R, Chenal C &Misset M 2004. Effects of low doses of short-term gamma irradiation on growth all development through two generations of Pisumsativum. Sci. Total Envt., 320: 121-129.