FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL

(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170

FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL

ANTIMICROBIAL ACTIVITIES OF SILVER NANOPARTICLES AGAINST SKIN DISEASE-ASSOCIATED OPPORTUNISTIC PATHOGENS
Pages: 437-441
Nejo, A.O., 2Obidi, O. F., 3Ayeni, R. A., 4Igwo Ezikpe, M. N


keywords: Antimicrobial activity, Green synthesis, Pathogens, Skin infection, Silver nanoparticles.

Abstract

Silver nanoparticles (AgNPs) is known to possess effective antimicrobial properties against a wide range of skin diseases. The use of green synthesis from plant extracts has also attracted attention because it is inexpensive, simple and environmentally friendly. This study focuses on evaluating the activity of silver nanoparticles on Staphylococcus epidermidus and Candida albicans which have been implicated in various cases of opportunistic infections of the skin. The potato capped silver nanoparticles were synthesised through a green route and characterised using UV, XRD, and TEM. Antimicrobial effect was compared with conventional tetracycline and nystatin using an agar well diffusion assay. The diameters of growth inhibition zones (ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) were assessed. For AgNPs, ZOI was 34mm and 42mm at 90mg/mL while MIC and MBC/MFC were 5.63mg/ml and 2.82mg/mL for S. epidermidus and C. albicans respectively. Comparatively, ZOI of tetracycline and nystatin were 41mm and 18mm at 90mg/mL while MIC and MBC/MFC were <3.13 mg/mL and > 100mg/mL for S. epidermidus and C. albicans respectively. AgNPs therefore had a better performance on C. albicans than the conventional nystatin. The Ultra-violet visible Spectrophotometer showed absorbance peak at 520 nm, the Transmission Electron Microscopy showed pentagonal AgNPs with an average particle size of 76 nm while the XRD shows the crystalinity of the NPs and also confirms the nanoparticles possessing the face centred cubic structure (fcc). The results of this study gives credence to the scientific basis of AgNPs for skin care formulations.

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